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light sheet microscopy  (Olympus)


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    Structured Review

    Olympus light sheet microscopy
    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
    Light Sheet Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 2347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+sheet+microscope/MVX10+Macro+Zoom+Fluorescence+Microscope+System/bio_rxiv__2025__11__20__689634-64-7-10
    Average 96 stars, based on 2347 article reviews
    light sheet microscopy - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex"

    Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

    Journal: bioRxiv

    doi: 10.1101/2025.11.20.689634

    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
    Figure Legend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Techniques Used: Immunohistochemical staining, Expressing, Fluorescence, Microscopy

    Related Articles

    Microscopy:

    Article Title: Voltage indicators
    Article Snippet: .. The zebrafish was further mounted to a custom-made chamber using 2% agarose (Sigma-Aldrich, A9414) and placed under a light-sheet microscope (45) with a 20× objective lens (Olympus, XLUMPLFLN). ..

    Article Title: Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation
    Article Snippet: .. To evaluate the colocalization analysis using the light-sheet microscope, selected cortical regions of the cleared brain were also imaged using a confocal microscope (BX61WI, Olympus) equipped with a 25X objective lens (NA: 1.00, WD: 8 mm, Olympus) and 559- and 635-nm lasers (NTT Electronics). ..

    Article Title: Optical sectioning methods in three-dimensional bioimaging
    Article Snippet: .. Based on the optical sectioning model mentioned earlier and the assumption of ideal illumination, the optical sectioning strength of specific techniques can be represented by the FWHM of Eq. , as shown in Fig. . \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$I(u)=\left\{\begin{array}{l}\int {T}^{2}(s,u)jinc(\beta s)sds\,{\rm{Confocal}}\\ \int {T}^{2}({s}_{x},0,u)\sin c(\beta {s}_{x}/\pi )d{s}_{x}\,{{\rm{Line}}\,{\rm{Confocal}}}\\ \int T(s/2,u/2)T(s,u)sds\,{\rm{Two}}-{\rm{photon}}\\ \exp (-{u}^{2}/2{c}^{2})T({s}_{x},{s}_{y},u)\delta ({s}_{x},{s}_{y})\,{{\rm{Light}}\,{\rm{sheet}}}\\ \int \delta ({s}_{x}-{{\rm{s}}}_{0})T({s}_{x},0,u)d{s}_{x}\,{{\rm{Structured}}\,{\rm{illumination}}}\\ \int {T}^{2}({s}_{x},0,u)\sin c(\beta {s}_{x}/\pi )[1-\,\cos (2\beta {s}_{x})]d{s}_{x}\,{{\rm{Natural}}\,{\rm{illumination}}}\end{array}\right.$$\end{document} I ( u ) = ∫ T 2 ( s , u ) j i n c ( β s ) s d s Confocal ∫ T 2 ( s x , 0 , u ) sin c ( β s x / π ) d s x Line Confocal ∫ T ( s / 2 , u / 2 ) T ( s , u ) s d s Two − photon exp ( − u 2 / 2 c 2 ) T ( s x , s y , u ) δ ( s x , s y ) Light sheet ∫ δ ( s x − s 0 ) T ( s x , 0 , u ) d s x Structured illumination ∫ T 2 ( s x , 0 , u ) sin c ( β s x / π ) [ 1 − cos ( 2 β s x ) ] d s x Natural illumination where the light sheet microscope employs a 20× detection objective (NA 1.0, XLUMPLFLN 20XW, Olympus) and a 25× illumination objective (NA 1.0, XLSLPLN25XGMP, Olympus) with a working distance of 8 mm. ..

    Article Title: A zebrafish rbm24a-GFP knock-in line for monitoring lineage-specific dynamic protein expression and function.
    Article Snippet: The RNA-binding protein Rbm24 is evolutionarily conserved, and its coding gene displays tissue-specific expression in vertebrates.. However, the dynamic localization of this protein in different cell lineages remains elusive.. We have generated a zebrafish rbm24a-GFP knock-in line in which endogenous Rbm24a is tagged with GFP, allowing the precise monitoring and systematic characterization of its spatiotemporal expression and subcellular localization during development and in the adult.

    Article Title: Restoration of locomotor function following stimulation of the A13 region in Parkinson’s mouse models
    Article Snippet: .. Cleared whole brain samples were imaged using a light-sheet microscope (LaVision Biotech UltraMicroscope, LaVision, Bielefeld, Germany) with an Olympus MVPLAPO 2x objective with 4x optical zoom (NA = 0.475) and a 5.7 mm dipping cap that is adjusted for the high refractive index of 1.56. ..

    Article Title: A xenotransplantation mouse model to study physiology of the mammary gland from large mammals
    Article Snippet: To finish clearing and in preparation for light sheet imaging, MFPs were embedded in Ultrapure Agarose (1.5% (w/v)) (cat #:16500–500) (Invitrogen) in CUBIC Reagent 2 within polymethylmethacrylate square spectrophotometer cuvettes (cat #: 111157) (Globe Scientific, Mawah, NJ). .. Fluorescent images were taken using a Light Sheet microscope (LaVision Biotech, Bielefeld, Germany) designed around an Olympus MVX-10 zoom macroscope (Olympus). .. Xenograft tissues were imaged using wavelengths at 488 nm (Alexa Fluor TM 488 Phalloidin) and 561 nm (autofluorescence) and 3D renderings and z-stack (10 μm optical thickness) videos were generated using Arivis Vision 4D software (version 3.4) (Zeiss, Dublin, CA).

    Article Title: Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation.
    Article Snippet: .. To evaluate the colocalization analysis using the light-sheet microscope, selected cortical regions of the cleared brain were also imaged using a confocal microscope (BX61WI, Olympus) equipped with a 25X objective lens (NA: 1.00, WD: 8mm, Olympus) and 559- and 635-nm lasers (NTT Electronics). ..

    Article Title: Voltage imaging reveals circuit computations in the raphe underlying serotonin-mediated motor vigor learning
    Article Snippet: .. The zebrafish was further mounted to a custom-made chamber using 2% agarose (Sigma-Aldrich, A9414) and placed under a light-sheet microscope with a 20x objective lens (Olympus, XLUMPLFLN). ..

    Imaging:

    Article Title: A zebrafish rbm24a-GFP knock-in line for monitoring lineage-specific dynamic protein expression and function.
    Article Snippet: The RNA-binding protein Rbm24 is evolutionarily conserved, and its coding gene displays tissue-specific expression in vertebrates.. However, the dynamic localization of this protein in different cell lineages remains elusive.. We have generated a zebrafish rbm24a-GFP knock-in line in which endogenous Rbm24a is tagged with GFP, allowing the precise monitoring and systematic characterization of its spatiotemporal expression and subcellular localization during development and in the adult.

    Refractive Index:

    Article Title: Restoration of locomotor function following stimulation of the A13 region in Parkinson’s mouse models
    Article Snippet: .. Cleared whole brain samples were imaged using a light-sheet microscope (LaVision Biotech UltraMicroscope, LaVision, Bielefeld, Germany) with an Olympus MVPLAPO 2x objective with 4x optical zoom (NA = 0.475) and a 5.7 mm dipping cap that is adjusted for the high refractive index of 1.56. ..



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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Image Search Results


    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Journal: bioRxiv

    Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

    doi: 10.1101/2025.11.20.689634

    Figure Lengend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Article Snippet: Cleared whole brain tissues were imaged by Light sheet microscopy (Olympus, MVX10-LS), and the z-axis interval was 4 μm in the P0 sample and 9 μm in the 8 weeks.

    Techniques: Immunohistochemical staining, Expressing, Fluorescence, Microscopy